Journal: Molecular Therapy

Article Title: CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

doi: 10.1016/j.ymthe.2020.04.003

Figure Lengend Snippet: GMSCs Contribute to Bone Formation after Injection into Mice (A and B) A 50-μL Matrigel plug containing 1 × 10 5 GMSCs was implanted subcutaneously in immunocompromised mice. (A) Schematic of the transplantation model. (B) Representative gross look of subcutaneous tissue and micro-CT images illustrating organoids in a transplantation model. The quantification of bone volumes in subcutaneous tissues, n = 3. (C–E) 2 × 10 6 of GMSCs were fluorescently labeled with DiR and adoptively infused into C57BL/6 mice. (C) Schematic of the GMSC tracking model. (D) Representative digital IVIS images in different groups on 3 days after injection, n = 3. (E) Representative digital IVIS images and quantification of fluorescence intensity in different time points, n = 3. (F) GMSCs and osteoblast precursors (OBPs) cocultured (GMSCs to OBPs = 1:10) in the Transwell system; then induced osteogenesis followed with alizarin red staining. Representative gross look and image under microscope were shown under different conditions and the quantification by hexadecyl pyridinium chloride monohydrate in different treatment groups n = 3. The results represent three independent experiments (mean ± SEM), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by Mann-Whitney tests or t test or ANOVA test.

Article Snippet: A 50-μL Matrigel plug (Corning Matrigel Growth Factor Reduced Basement Membrane Matrix, 356231) containing 1 × 10 5 GMSCs was implanted subcutaneously, and the contralateral site was injected with a Matrigel plug as a control.

Techniques: Injection, Transplantation Assay, Micro-CT, Labeling, Fluorescence, Staining, Microscopy, MANN-WHITNEY

Journal: Cell reports

Article Title: TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling

doi: 10.1016/j.celrep.2019.07.086

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Corning Matrigel Matrix - High , Fisher Scientific , Cat# 354248.

Techniques: Virus, Generated, Recombinant, Reverse Transcription, Saline, Cloning, Gel Extraction, Western Blot, Stripping, Bicinchoninic Acid Protein Assay, shRNA, Software, SDS-Gel

Journal: STAR Protocols

Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells

doi: 10.1016/j.xpro.2021.100683

Figure Lengend Snippet: Direct differentiation of hPSC-derived airway epithelial organoids from basal cell reporter iPSCs, BU3-NGPT iPSCs (A) Genomic structure of the basal cell reporter in BU3-NGPT iPSCs. eGFP gene is integrated in both alleles of NKX2-1 and tdTomato gene is integrated in one allele of TP63 . (B) Schematic of the airway-directed differentiation protocol detailed in McCauley et al. 2018 . Predicted fluorescence protein expression patterns from the basal cell reporter are shown in color. (C) Representative image demonstrating GFP and tdTomato fluorescence expression in BU3-NGPT on day 15 (upper panels) or day 29 (lower panels) of differentiation. Scale bar = 100 μm Representative BU3-NGPT culture in one Matrigel droplet on day 29 of differentiation (10× magnification, 6 × 6 tile scan).

Article Snippet: Matrigel hESC-Qualified Matrix , Corning , Cat.# 354277.

Techniques: Derivative Assay, Fluorescence, Expressing

Journal: STAR Protocols

Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells

doi: 10.1016/j.xpro.2021.100683

Figure Lengend Snippet: Examination and isolation of iBCs (A) Representative image of organoid on day 40 of differentiation showing GFP and tdTomato expression (left panel) and its morphology (middle panel). Representative BU3-NGPT culture in one Matrigel droplet on day 40 of differentiation (Right panel, 10× magnification, 7 × 7 tile scan). (B) Representative expression pattern of NGFR and EpCAM (left panel) or GFP and tdTomato (middle panel) in single live cells of BU3-NGPT on day 40. iBCs are gated first for NKX2-1 GFP+ and TP63 tdTomato+ population in bold line square (middle panel) and then sorted for NGFR + EpCAM + population in bold line square (right panel). (C) Representative expression pattern of NGFR and EpCAM (left panel) in single live cells of non-reporter iPSC on day 44. The suggested gating for NGFR + EpCAM + iBC population is shown with bold line square.

Article Snippet: Matrigel hESC-Qualified Matrix , Corning , Cat.# 354277.

Techniques: Isolation, Expressing

Journal: STAR Protocols

Article Title: Differentiation of human pluripotent stem cells into functional airway basal stem cells

doi: 10.1016/j.xpro.2021.100683

Figure Lengend Snippet:

Article Snippet: Matrigel hESC-Qualified Matrix , Corning , Cat.# 354277.

Techniques: Control, Recombinant, Software, Membrane, Gentle, Modification, Blocking Assay, Plasmid Preparation, Microscopy

Journal: bioRxiv

Article Title: C-type lectin-like receptor 2 (CLEC-2)-dependent DC migration is controlled by tetraspanin CD37

doi: 10.1101/227918

Figure Lengend Snippet: (A) WT (left), Cd37-/- (middle) or CD11c ΔCLEC”2 (right) DCs were stimulated in a 3D collagen gel with (bottom row) or without (upper row) recombinant podoplanin-Fc (rPDPN-Fc). Cells were stained for F-actin (red) and nucleus (blue) and imaged with a Leica SP5 confocal fluorescence microscope. One representative cell is shown for each condition (overview with more cells is provided in Supplementary Figure 2). Scale bar = 10μm. (B-D) Number (B) and length (C) of actin protrusions, and morphology index (D) of WT (left), Cd37-/- (middle) or CD11c ΔCLEC ” 2 (right) BMDCs upon rPDPN-Fc stimulation (grey boxes) compared to no ligand (white boxes). Data are shown as Tukey Box & whiskers from 3 independent experiments, total n=3 mice per genotype. In Tukey Box & whiskers, black dots are determined as outliers; i.e. data points outside the 25 th and 75 th percentile, minus or plus the 1.5 interquartile range, respectively. Two-way ANOVA with Tukey’s multiple comparisons, ***p<0.001, ****p<0.0001.

Article Snippet: BMDCs (0.3x10 6 ) were seeded into a 3D collagen (type I, rat tail)/matrigel matrix (both from Corning, via Thermo Fisher Scientific, Loughborough, UK) supplemented with 10% minimum essential medium alpha medium (MEMalpha, Invitrogen, via Thermo Fisher Scientific, Loughborough, UK) and 10% FCS (Greiner Bio-One, Stonehouse, UK) on glass-bottomed cell culture plates (MatTek Corporation, Bratislava, Slovakia).

Techniques: Recombinant, Staining, Fluorescence, Microscopy

Journal: Cell Reports Medicine

Article Title: Co-targeting RANK pathway treats and prevents acquired resistance to CDK4/6 inhibitors in luminal breast cancer

doi: 10.1016/j.xcrm.2023.101120

Figure Lengend Snippet:

Article Snippet: Phenol-free matrigel solution , Corning® , 356237.

Techniques: Recombinant, Control, shRNA, Blocking Assay, Alamar Blue Assay, Flow Cytometry, Isolation, cDNA Synthesis, Bicinchoninic Acid Protein Assay, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Gene Expression, Western Blot, Software